NanoLab 3D™
Error-free DLS particle sizing even at high concentration
The NanoLab 3D™ is a compact instrument for DLS particle sizing based on the groundbreaking and patented Modulated 3D Cross-Correlation technology. It efficiently suppresses multiple light scattering, so sample dilution is no longer required for most samples.
Conventional DLS instruments carry the risk that multiple scattered light can lead to incorrect particle size measurements without ever warning you. Especially with concentrated samples, complex sample preparation and dilution are necessary. This is exactly where our NanoLab 3D comes in.
What can it measure?
- Particle size
- Polydispersity
- Viscosity
Your benefits
- Error-free measurement with modulated 3D technology
- No dilution of samples required
- Best measurement sensitivity on the market
- Characterize multimodal samples with our AI-powered CORENN algorithm
Small volumes
As little as 4μL of sample requiredFast
Easy-to-use
Support
Our team of experts is here to answer your questionsFeatures
- Advanced DLS particle sizing: Cumulant and CORENN analysis
- DLS microviscometry
- Applicable to diluted as well as highly concentrated samples
- Small sample volume - down to 4 µL (optional)
- Particle sizing from 0.15 nm to 5 µm radius
- Temperature range: 4°C to 85°C *
- Monitors time-dependent processes
- Compact and robust design
- Online support for all customers
- Conforms to ISO 22412
* Climate controlled room at or below 23°C required to meet these specifications
Dynamic Light Scattering (DLS)
Dynamic light scattering is the technology of choice to measure the size of nanoparticles dispersion. Driven by Brownian motion, the particles move within the medium, causing the intensity of scattered light to fluctuate. The statistics of these fluctuations are reflected in the correlation function. Since the size of the particles influences the particle movement and thus the statistics, DLS can extract the particle size distribution from the obtained correlation function.
Can you trust your measurements?
Unlike standard DLS instruments, the NanoLab 3D uses two laser beams which are modulated at very high frequency. The instrument thus performs two light scattering experiments simultaneously. By cross-correlating the signals of the two experiments, we improve precision by eliminating multiple scattering that otherwise causes an undetectable error in all samples that are not substantially diluted! Only the Modulated 3D technology fully removes multiple scattering arising in concentrated samples, allowing measurements up to the highest concentrations guaranteeing the trustworthiness of the measurement.
Zero-shear viscosity
With the Modulated 3D technology, it is also possible to determine the zero shear viscosity by measuring the diffusion of tracer particles added to the sample. The measured viscosity ranges from about 0.01 cP to 1000 cP and higher! DLS microviscometry is applicable to a wide range of samples and the NanoLab 3D is particularly powerful for characterising highly concentrated protein solutions, using extremely low volumes, without external pertubations to the sample and no detrimental surface effects.
Powerful analytical tools
We designed the software of the NanoLab 3D for both DLS experts as well as beginners without specific training. Anything from one simple, yet reliable measurement, to a complex series of multiple measurements can be performed within just a few clicks. Powerful analytical tools allow highly customizable data display and export while saving all results in a well-organized and comprehensive database. Analytical tools included are Cumulant and the LSI proprietary algorithm CORENN.
AI-powered algorithm
CORENN is a novel advanced machine learning algorithm to extract the particle size distribution (PSD) from a DLS measurement. CORENN is the only DLS inversion algorithm that leverages on advanced signal approximation techniques and a unique theoretical estimate of the signal noise to yield extremely reliable results, robust against experimental distortions, thus enabling the end-user to obtain the true PSD from real world DLS experiments. The figure on the left shows a DLS measurement of a particle mixture of 4 nm and 45 nm. Only CORENN is able to correctly determine the two populations.
Control Your Process with the Stop-Flow Cell Option
The Stop-Flow Cell is available as a separate option and can be connected to an external circuit via Idex 6-32 Flat-Bottom connectors for use with 1/16'' OD piping. Due to the modulated 3D technology for dilution-free DLS measurements, the NanoLab 3DTM coupled with this Stop-Flow Cell allows for the first time the use of DLS for at-line process control.
Specifications
| Technology | Modulated 3D cross-correlation DLS (EU Patent) |
| Hydrodynamic radius | 0.15 nm-5μm* |
| Cuvette sizes | 3 x 3 mm, 5 x 5 mm, 10 x 10 mm (optional 1 x 1 mm) |
| Maximum concentration | 40 % w/v* |
| Sample volume | 50 μL —2 mL (optional 4 μL) |
| Temperature range | 4° C to 85° C (+/-0.02 °C)** measured online |
| Laser class | 1 |
| Wavelength | 638 nm |
| Laser power | 120 mW |
| Scattering angle | 90° |
| Detection | Single mode fiber with high performance APD. QE 65 % |
| Correlator |
Two channel multiple tau, 12.5 ns -1 h, 320 channels |
| Laser attenuator | System with online incident power measurement |
| Software | Including Cumulant, CONTIN, CORENN analysis |
| Laboratory requirements | < 60 % relative humidity and T = 17 to 26 °C |
| Size | 45 x 45 x 30 cm |
| Weight | Approx. 30 kg |
* As for all DLS instruments, the maximum range is sample dependent.
** A climate controlled room at or below 23 °C required to meet these specifications, for temperatures below the dew point a dry air source is required.